Protein Purification

Preface to The Third Edition vii

Preface to The Second Edition ix

Preface to The First Edition xi

Contributors xiii

Part I Introduction 1

1 Introduction to Protein Purification 3
Bo Ersson, Lars Ryden, and Jan-Christer Janson

Part II Chromatography 23

2 Introduction to Chromatography 25
Jan-Christer Janson and Jan Ake Joensson

3 Gel Filtration: Size Exclusion Chromatography 51
Lars Hagel

4 Ion Exchange Chromatography 93
Evert Karlsson and Irwin Hirsh

5 High-Resolution Reversed-Phase Chromatography of Proteins 135
Sylvia Winkel Pettersson

6 Hydrophobic Interaction Chromatography 165
Kjell-Ove Eriksson and Makonnen Belew

7 Immobilized Metal Ion Affinity Chromatography 183
Lennart Kagedal

8 Covalent Chromatography 203
Francisco Batista-Viera, Lars Ryden, and Jan Carlsson

9 Affinity Chromatography 221
Francisco Batista-Viera, Jan-Christer Janson, and Jan Carlsson

10 Affinity Ligands from Chemical Combinatorial Libraries 259
Enrique Carredano and Herbert Baumann

11 Affinity Ligands from Biological Combinatorial Libraries 269
Per- Ake Nygren

Part III Other Separation Methods and Related Techniques 279

12 Membrane Separations 281
Joachim K. Walter, Zuwei Jin, Maik W. Jornitz, and Uwe Gottschalk

13 Refolding of Inclusion Body Proteins from E. coli 319
Zhiguo Su, Diannan Lu, and Zheng Liu

14 Purification of PEGylated Proteins 339
Conan J. Fee and James M. Van Alstine

Part IV Electrophoresis 363

15 Electrophoresis in Gels 365
Reiner Westermeier

16 Conventional Isoelectric Focusing in Gel Slabs and Capillaries and Immobilized pH Gradients 379
Pier Giorgio Righetti, Elisa Fasoli, and Sabina Carla Righetti

17 Two-Dimensional Electrophoresis in Proteomics 411
Reiner Westermeier and Angelika Goerg

18 Protein Elution and Blotting Techniques 441
Reiner Westermeier

19 Capillary Electrophoretic Separations 451
Wolfgang Thormann

Part V Separation Method Optimization 487

20 High Throughput Screening Techniques in Protein Purification 489
Karol M. Lacki and Eggert Brekkan

Index 507

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